mRNA profiling zur Bestimmung der Spurenart

Körpersekrete werden herkömmlich mit enzymatischen und immunologischen Vortests nachgewiesen, die zum Teil unspezifisch sind. Mittels mRNA profiling wird die Expression sekretspezifischer Proteine nachgewiesen. Innerhalb der EDNAP Gruppe (European DNA profiling group) wurden mRNA Marker für Blut, Speichel, Sperma, Vaginalsekret, Menstrualblut und Haut getestet. Mittels Hochdurchsatzsequenzierung suchen wir innerhalb der mRNA-Transkripte nach Sequenzvarianten, die es erlauben das Sekret dem Spurengeber zuzuordnen.

University of Central Florida, USA

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• Hanson E, Ingold S, Haas C, Ballantyne J. Messenger RNA biomarker signatures for forensic body fluid identification revealed by targeted RNA sequencing. Forensic Sci Int Genet 34 (2018) 206-221.
• Haas C, et al. RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise. Forensic Sci Int Genet 16 (2015) 139–47.
• Haas C, et al. RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise. Forensic Sci Int Genet 8 (2014) 203–212.
• Haas C, et al. RNA/DNA co-analysis from human saliva and semen stains – results of a third collaborative EDNAP exercise. Forensic Sci Int Genet 7 (2013) 230-239.